聚乙烯醇共混改性膜作为农药种衣剂成膜剂的性能研究

以水溶性聚合物聚乙烯醇(PVA)和水不溶性聚合物乙酸乙烯-乙烯共聚物(VAE)乳液为原料,采用溶液共混法制备了PVA-VAE共混膜,为农药成膜剂的制备开辟了新途径。红外光谱、透射电镜、激光粒度仪测试结果显示,PVA溶液中的胶束被打开,体系的比表面积急剧增大,两相间的界面消失,说明共混体系分子间具有良好的相容性;透水性与耐水性测定结果表明,PVA-VAE的质量比为80∶ 20、70∶ 30、60∶ 40时其共混膜的性能适用于农药种衣剂的成膜剂;种子发芽试验显示,与单独使用PVA膜相比,引入适当比例VAE后的共混膜用于种子包衣,其耐水性显著提高,渗水率适宜,对种子发芽无明显影响。     

猪肺炎支原体膜蛋白P46基因在大肠杆菌中的表达

把猪肺炎支原体(Mycoplasma hyopneumoniae)国际标准株232的P46基因克隆进pGEM-T-EASY载体上,通过PCR方法把该基因中三个编码Trp的密码子TGA突变为TGG,然后将该基因亚克隆到载体pMAL-P2X上,得到重组表达载体pMAL-P2X-P46.用该重组载体转化大肠杆菌TB1,得到表达重组菌TB1-pMAL-P2XA-P46,用终浓度为0.3 mM的IPTG在37℃下诱导表达,获得可溶性表达的融合蛋白MBP-P46,在免疫印迹试验中,兔抗MBP高免血清和兔抗猪肺炎支原体高免血清都能与目的蛋白发生阳性反应,证明猪肺炎支原体P46基因在大肠杆菌里获得了可溶性表达.该融合蛋白对于建立特异性和敏感性好的EIISSA方法具有重要意义.     

小麦品种在低温下膜功能变化的生物物理学分析

本文以冬性及春小麦品种为试材，用示踪，液闪计数及测定电解质泄漏的方法，测定低温胁迫下膜脂的代谢和电解质渗透的变化，并根据热力学的原理，分析其生物膜上的化学反应和扩散过程及膜上物质通量Ｊｊ与跨膜电势Ｅｍ的函数关系。     

巢湖市“五早”作物生产可持续发展的思考

针对巢湖市"五早"作物生产的实施成效和面临的形势与问题,从优质高产、市场流通、配套服务等多方面提出了可持续发展的"三大"转变措施.     

布鲁菌外膜蛋白及毒力因子研究进展

布鲁菌细胞膜的基本结构包括脂多糖和外膜蛋白,与细菌的毒力及免疫原性相关。文章描述了布鲁菌外膜蛋白分子结构的最新进展。该菌的外膜蛋白由第一组、第二组和第三组外膜蛋白构成。第一组外膜蛋白对维持布鲁菌外膜蛋白的结构起重要作用;第二组包括36 ku~38 ku外膜蛋白,为膜孔蛋白,由Omp2a和Omp2b基因编码,其中38 ku蛋白基因可能是一个与毒力相关的基因;第三组外膜蛋白包括31 ku和25 ku两个相关的蛋白,具有重要的免疫功能。31 ku蛋白属膜孔蛋白,25 ku蛋白还与毒力有关。文章也介绍了布鲁菌毒力因子研究的最新进展。     

La(NO3)3对4个苜蓿品种种子活力与膜透性作用的比较

通过La3 离子对4个苜蓿Medicago sativa品种种子活力与膜透性作用的比较,结果表明:当用8种不同浓度La(NO3)3溶液处理4个苜蓿品种种子时,La3 浓度小于600μg/mL时,可促进种子的活力;其中,中苜1号和胜利者的活力指数变化稳定且较高,而草原1号在La3 浓度为600μg/mL时变化剧烈。中苜1号和胜利者可以较长时间忍耐La3 在750μg/mL的处理浓度,超过此剂量时,膜结构就受到了破坏;而阿尔冈金和草原1号在La3 浓度接近750μg/mL时,种子活力就已经开始下降了。     

ULTRASONOGRAPHIC EVALUATION OF THE EXTERNAL EAR CANAL AND TYMPANIC MEMBRANE IN DOGS

Ultrasonographic imaging of the canine external ear canal, tympanic membrane, and tympanic bulla was described in five healthy beagle dogs before and after infusion of saline into the ear canal. Saline served as an acoustic window. With this method, the external ear canal, and tympanic bulla were visible in the same imaging plane and the integrity of the tympanic membrane could be evaluated indirectly by confirming an intact tympanic membrane, which appeared at the end of the ear canal as a hyperechoic line with reverberation. Experimentally, perforated tympanic membrane could be evaluated by identifying anechoic saline in the tympanic bulla lumen. The air and fluid-filled tympanic bulla were also visualized. Ultrasonography with saline as an acoustic window appears to be helpful for the evaluation of the external ear canal, tympanic membrane, and tympanic bulla and it may have the potential to be a useful clinical tool in evaluation of integrity of the tympanic membrane.     

人工模拟高温环境下白三叶草相对生理指标测定

研究了高温胁迫条件下白三叶叶片相对含水量、膜透性、脯氨酸含量和叶绿素含量的变化规律。结果表明:25~35℃处理0.5 h后,白三叶的膜透性、脯氨酸含量逐渐上升,相对含水量、叶绿素含量逐渐下降,但幅度不大;45℃以上高温处理后,以上生理指标均有显著性大幅度的变化。与CK比较,温度每升高5℃,白三叶细胞质膜透性、脯氨酸含量平均增加50.62%、59.89%,而相对含水量、叶绿素含量分别下降34.22%、4.61%。在实验条件下,白三叶能适应35℃的高温,40~45℃为其忍耐高温的临界温度范围,50℃为致死温度上限。     

The Application and Research Progress of Chicken Embryo and Avian Cell Lines in Biomedicine

Chicken embryo has long been an important experimental model in basic and applied science because of its clear development process,especially in the early development of chicken embryo chorioallantoic membrane stage,due to its abundant blood vessels,it is a natural immunodeficiency host and can be used as an ideal experimental model for pathology,pharmacology and oncology research.The authors briefly described the tissue structure of early stage of chicken embryo,the application of chick embryo chorioallantoic membrane model in tumor,angiogenesis,organ transplantation,burn and other diseases,and the application of anti-cancer drug screening based on the pathological model of chicken embryo.The advances in the application of chicken embryo and avian cell lines in virus reproduction,vaccine production,therapeutic protein production and monoclonal antibody production were reviewed.A variety of human viruses,avian viruses and mycoplasma can proliferate in chicken embryos and avian cell lines and be used in vaccine production.In this paper,the development and characteristics of commonly used avian fibroblasts and pluripotent stem cells were described,and the source of commercial avian cell lines and some susceptible viruses were summarized.Chicken embryo expression system can produce human glycosylates at specific sites of target proteins,reduce the allergic reaction of the target protein to human,and poultry eggs are cheap and easily available,so it can be used as a suitable donor for the production of human monoclonal antibodies and therapeutic proteins.In this paper,the recent progress in the application of chicken embryos and avian cell lines in the field of biomedicine was introduced,and the future application of chicken embryos as animal models was forecasted.     

内毒素对兔红细胞膜磷脂含量的影响及阳离子A对其的拮抗效应

利用高效液相色谱法分别测定正常对照组（Ⅰ组）、内毒素组（Ⅱ组）和内毒素＋阳离子A拮抗组（Ⅲ组）中兔红细胞膜磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、磷脂酰丝氨酸（PS）和磷脂酰肌醇（PI）含量。结果显示，Ⅱ组中除PE在处理后0．5h没有明显变化外（P〉0．05），PC、PE、PS、PI含量在0，5、1、3、5、7h均低于Ⅰ组（P〈0.05，P〈0．01）；而Ⅲ组中除PS、PE和PI在处理后0．5h、PC在1h、PI在3h没有明显变化外（P〉0．05），整个时间段中上述4种磷脂的含量均高于Ⅱ组（P〈0．05，P〈0．01）。可见，内毒素能使兔红细胞膜磷脂含量降低，而阳离子A在一定程度上具有对内毒素的拮抗作用和对红细胞膜的保护效应。